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Clapper ML , Szarka CE , Pfeiffer GR , Graham TA , Balshem AM , Litwin S , Goosenberg EB , Frucht H , Engstrom PF
Preclinical and clinical evaluation of broccoli supplements as inducers of glutathione S-transferase activity
Clinical Cancer Research. 1997 Jan;3(1) :25-30
AbstractPrevious studies suggest that cruciferous vegetables may provide protection against carcinogen exposure by inducing detoxification enzymes, ICR(Ha) mice were gavaged with broccoli tablets (1 g/kg), and colon tissues were collected after treatment, Glutathione S-transferase (GST) activity was assayed and peaked on days 1 and 2 after treatment, respectively (P = 0.03), Elevations in GST activity were attributed to the increased expression of mu and pi. These data supported a clinical assessment of broccoli supplements, Twenty-nine subjects at increased risk for colorectal cancer were randomized to group 1 (no cruciferous vegetables) or group 2 (broccoli supplements, 3 g/day) for 14 days, Blood samples and colon biopsies were obtained pre- and postintervention, No significant difference was observed between the GST activities of the control and broccoli supplementation groups posttreatment, Mean lymphocyte GST activity was 107% of baseline in the broccoli supplementation group (range, 79-158%) and 102% of baseline in the control group (range, 75-158%), Correlation of the GST activities of blood lymphocytes and colon mucosa taken simultaneously suggested that the GST activity of blood lymphocytes may be used as a biomarker of the responsiveness of colon tissue to chemopreventive regimens, Future clinical studies evaluating cruciferous vegetables should consider using concentrated dietary supplements in subjects with a previous history of colorectal cancer.
NotesTimes Cited: 7 English Article WY363 CLIN CANCER RES