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Ratushny V , Golemis E
Resolving the network of cell signaling pathways using the evolving yeast two-hybrid system
Biotechniques. 2008 Apr;44(5) :655-62
PMID: 18474041    PMCID: PMC2526548   
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In 1983, while investigators had identified a few human proteins as important regulators of specific biological outcomes, how these proteins acted in the cell was essentially unknown in almost all cases. Twenty-five years later, our knowledge of the mechanistic basis of protein action has been transformed by our increasingly detailed understanding of protein-protein interactions, which have allowed us to define cellular machines. The advent of the yeast two-hybrid (Y2H) system in 1989 marked a milestone in the field of proteomics. Exploiting the modular nature of transcription factors, the Y2H system allows facile measurement of the activation of reporter genes based on interactions between two chimeric or "hybrid" proteins of interest. After a decade of service as a leading platform for individual investigators to use in exploring the interaction properties of interesting target proteins, the Y2H system has increasingly been applied in high-throughput applications intended to map genome-scale protein-protein interactions for model organisms and humans. Although some significant technical limitations apply, Y2H has made a great contribution to our general understanding of the topology of cellular signaling networks.
Ratushny, Vladimir Golemis, Erica CA-06927/CA/NCI NIH HHS/United States CA113342/CA/NCI NIH HHS/United States CA63366/CA/NCI NIH HHS/United States P30 CA006927-45/CA/NCI NIH HHS/United States R01 CA063366-13/CA/NCI NIH HHS/United States R01 CA113342-01A2/CA/NCI NIH HHS/United States Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review England BioTechniques Biotechniques. 2008 Apr;44(5):655-62.