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Pannier AK , Ariazi EA , Bellis AD , Bengali Z , Jordan VC , Shea LD
Bioluminescence imaging for assessment and normalization in transfected cell arrays
Biotechnology and Bioengineering. 2007 Oct 1;98(2) :486-97
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Abstract
Transfected cell arrays (TCAs) represent a high-throughput technique to correlate gene expression with functional cell responses. Despite advances in TCAs, improvements are needed for the widespread application of this technology. We have developed a TCA that combines a two-plasmid system and dual-bioluminescence imaging to quantitatively normalize for variability in transfection and increase sensitivity. The two-plasmids consist of: (i) normalization plasmid present within each spot, and (ii) functional plasmid that varies between spots, responsible for the functional endpoint of the array. Bioluminescence imaging of dual-luciferase reporters (renilla, firefly luciferase) provides sensitive and quantitative detection of cellular response, with minimal post-transfection processing. The array was applied to quantify estrogen receptor alpha (ERalpha) activity in MCF-7 breast cancer cells. A plasmid containing an ERalpha-regulated promoter directing firefly luciferase expression was mixed with a normalization plasmid, complexed with cationic lipids and deposited into an array. ER induction mimicked results obtained through traditional assays methods, with estrogen inducing luciferase expression 10-fold over the antiestrogen fulvestrant or vehicle. Furthermore, the array captured a dose response to estrogen, demonstrating the sensitivity of bioluminescence quantification. This system provides a tool for basic science research, with potential application for the development of patient specific therapies.
Notes
Pannier, Angela K Ariazi, Eric A Bellis, Abigail D Bengali, Zain Jordan, V Craig Shea, Lonnie D P50 CA 89018/CA/United States NCI R01 GM066830/GM/United States NIGMS Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. United States Biotechnology and bioengineering Biotechnol Bioeng. 2007 Oct 1;98(2):486-97.