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Rao A , Haywood J , Craddock AL , Belinsky MG , Kruh GD , Dawson PA
The organic solute transporter alpha-beta, Ost alpha-Ost beta, is essential for intestinal bile acid transport and homeostasis
Proceedings of the National Academy of Sciences of the United States of America. 2008 Mar;105(10) :3891-3896
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Abstract
The apical sodium-dependent bile acid transporter (Asbt) is responsible for transport across the intestinal brush border membrane; however, the carrier(s) responsible for basolateral bile acid export into the portal circulation remains to be determined. Although the heteromeric organic solute transporter Ost alpha-Ost beta exhibits many properties predicted for a candidate intestinal basolateral bile acid transporter, the in vivo functions of Ost alpha-Ost beta have not been investigated. To determine the role of Ost alpha-Ost beta in intestinal bile acid absorption, the Ost alpha gene was disrupted by homologous recombination in mice. Ost alpha(-/-) mice were physically indistinguishable from wild-type mice. In everted gut sac experiments, transileal transport of taurocholate was reduced by >80% in Ost alpha(-/-) vs. wild-type mice; the residual taurocholate transport was further reduced to near-background levels in gut sacs prepared from Ost alpha(-/-)Mrp3(-/-) mice. Th!
Notes
ISI Document Delivery No.: 273KZ Rao, Anuradha Haywood, Jamie Craddock, Ann L. Belinsky, Martin G. Kruh, Gary D. Dawson, Paul A. NATL ACAD SCIENCES