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Liang M , Ayanga B , Du S , Godwin AK , Hartsock JK , Evans SC
OvcaI, a candidate gene of the genetic modifier of Tp53, Mop2, affects mouse embryonic lethality
Genes Chromosomes & Cancer. 2008 Apr;47(4) :315-325
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In this study, we show genetic modifier genes of Tp53 that can exacerbate embryonic abnormalities. Using a mouse model in which CE/J mice were crossed with the Tp53-null 129/Sv (129-Trp53(tml) (Tyj)) mice, a subset of Tp53+/- and -/- male and female embryos died during gestation. Our hypothesis, based on the genotypes of survivors, is that two genetic modifiers and a Tp53 null allele lead to an increase in embryonic lethality. We previously identified a recessive modifier (MopI) from CE/J mice on chromosome II centromeric to Tp53. We have uncovered a dominant modifier (Mop2) from 129/Sv mice telomeric to Tp53. We discovered a polymorphic change (32IP -> 32IS) of Ovca I within the Mop2 locus of CE/J mice. This polymorphism increased both mRNA and protein levels of OVCAI in various tissues. CE/J primary cells cultured from different tissues proliferated more rapidly than 129/Sv cells. In addition, CE/J cells cycled while 129/Sv cells had a higher arrest in the G I phase. Tr!
Liang, Min Ayanga, Bernard Du, Shuhua Godwin, Andrew K. Hartsock, Jennifer K. Evans, Susan C. WILEY-LISS