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Beacham DA , Amatangelo MD , Cukierman E
Preparation of extracellular matrices produced by cultured and primary fibroblasts
Curr Protoc Cell Biol. 2007 Jan;Chapter 10 :Unit10 9
AbstractFibroblasts secrete and organize extracellular matrix (ECM), which provides structural support for their adhesion, migration, and tissue organization, besides regulating cellular functions such as growth and survival. Cell-to-matrix interactions are vital for vertebrate development. Disorders in these processes have been associated with fibrosis, developmental malformations, cancer, and other diseases. This unit describes a method for preparing a three-dimensional matrix derived from NIH-3T3 cells; the matrix is three-dimensions, cell and debris free, and attached to a two-dimensional culture surface. Cell adhesion and spreading are normal on these matrices. This matrix can also be compressed into a two-dimensional matrix and solubilized to study the matrix biochemically.
NotesBeacham, Dorothy A Amatangelo, Michael D Cukierman, Edna United States Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.] Curr Protoc Cell Biol. 2007 Jan;Chapter 10:Unit10.9.