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Chang J , Nie X , Chang HE , Han Z , Taylor J
Transcription of Hepatitis Delta Virus RNA by RNA Polymerase II
J Virol. 2007 Nov 21
PMID: 18032511   
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Previous studies have indicated that the replication of the RNA genome of hepatitis delta virus (HDV) involves redirection of RNA polymerase II (pol II), a host enzyme that normally uses DNA as template. However, there has been some controversy about whether in one part of this HDV RNA transcription, a polymerase other than pol II is involved. The present study applied a recently described cell system (293-HDV) of tetracycline-inducible HDV RNA replication to provide new data regarding the involvement of host polymerases in HDV transcription. Data generated with a nuclear run-on assay demonstrated that synthesis not only of genomic RNA but also that of its complement, the antigenome, could be inhibited by low concentrations of amanitin specific for pol II transcription. Subsequent studies used immuno-precipitation and rate-zonal sedimentation of nuclear extracts together with double immuno-staining of 293-HDV cells, in order to examine the associations between pol II and HDV RNAs, as well as the small delta antigen, an HDV-encoded protein known to be essential for replication. Findings include evidence that HDV replication is somehow able to direct the available delta antigen to sites in the nucleoplasm, almost exclusively co-localized with pol II in what others have described as transcription factories.
J Virol. 2007 Nov 21;.