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Hartman TR , Qian S , Bolinger C , Fernandez S , Schoenberg DR , Boris-Lawrie K
RNA helicase A is necessary for translation of selected messenger RNAs
Nat Struct Mol Biol. 2006 May 7;13(6) :509-16
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RNA helicase A (RHA) is a highly conserved DEAD-box protein that activates transcription, modulates RNA splicing and binds the nuclear pore complex. The life cycle of typical mRNA involves RNA processing and translation after ribosome scanning of a relatively unstructured 5' untranslated region (UTR). The precursor RNAs of retroviruses and selected cellular genes harbor a complex 5' UTR and use a yet-to-be-identified host post-transcriptional effector to stimulate efficient translation. Here we show that RHA recognizes a structured 5'-terminal post-transcriptional control element (PCE) of a retrovirus and the JUND growth-control gene. RHA interacts with PCE RNA in the nucleus and cytoplasm, facilitates polyribosome association and is necessary for its efficient translation. Our results reveal a previously unidentified role for RHA in translation and implicate RHA as an integrative effector in the continuum of gene expression from transcription to translation.
P01ca16058/ca/nci P30ca100730/ca/nci Journal Article Research Support, N.I.H., Extramural United States