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Marone M , Scambia G , Giannitelli C , Ferrandina G , Masciullo V , Benedetti-Panici P , Mancuso S
Analysis of cyclin E and CDK2 in ovarian cancer: Gene amplification and RNA overexpression
International Journal of Cancer. 1998 Jan 5;75(1) :34-39
PMID: ISI:000071237200006   
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Cyclins and their associated kinases (cdks) play a key role in controlling the cell cycle, a process whose disregulation can potentially lead to uncontrolled cell growth and hence to cancer, We have studied the role of both cyclin E and its associated kinase cdk2 in ovarian cancer. Primary, metastatic, recurrent and benign ovarian tumors were screened for cyclin E and cdk2 gene amplification. Cyclin E was shown to be amplified in 21% and cdk2 in 6.4% of the cases analyzed, Cyclin E and cdk2 RNA expression levels were determined by semi-quantitative RT-PCR analysis in a partially overlapping series of samples and compared to the expression levels of normal ovarian surface epithelial cells, Cyclin E RNA was overexpressed in 29.5% and cdk2 in 6.5% of ovarian tumors tested. We determined that in most cases gene amplification leads to higher RNA levels for cyclin E and that the overall levels of cyclin E and cdk2 RNA were correlated. We hypothesize that cyclin E and cdk2 are, in part co-regulated and that they may concur to ovarian tumor development. (C) 1998 Wiley-Liss, Inc.
Times Cited: 28 English Article YP060 INT J CANCER