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Huang HM , Feng J , Famulski J , Rattner JB , Liu ST , Kao GD , Muschel R , Chan GKT , Yen TJ
Tripin/hSgo2 recruits MCAK to the inner centromere to correct defective kinetochore attachments
Journal of Cell Biology. 2007 May;177(3) :413-424
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Abstract
hSgo2 (previously annotated as Tripin) was recently reported to be a new inner centromere protein that is essential for centromere cohesion (Kitajima et al., 2006). In this study, we show that hSgo2 exhibits a dynamic distribution pattern, and that its localization depends on the l and Aurora B kinases. hSgo2 is concentrated at the inner centromere of unattached kinetochores, but extends toward the kinetochores that are under tension. This localization pattern is reminiscent of MCAK, which is a microtubule depolymerase that is believed to be a key component of the error correction mechanism at kinetochores. Indeed, we found that hSgo2 is essential for MCAK to localize to the centromere. Delocalization of MCAK accounts for why cells depleted of hSgo2 exhibit kinetochore attachment defects that go uncorrected, despite a transient delay in the onset of anal Consequently, these cells exhibit a high frequency of lagging chromosomes when they enter anaphase. We confirmed that h!
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