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Chen XW , Wilson RM , Kubo H , Berretta RM , Harris DM , Zhang XY , Jaleel N , MacDonnell SM , Bearzi C , Tillmanns J , Trofimova I , Hosoda T , Mosna F , Cribbs L , Leri A , Kajstura J , Anversa P , Houser SR
Adolescent feline heart contains a population of small, proliferative ventricular myocytes with immature physiological properties
Circulation Research. 2007 Mar;100(4) :536-544
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Recent studies suggest that rather than being terminally differentiated, the adult heart is a self-renewing organ with the capacity to generate new myocytes from cardiac stem/progenitor cells (CS/PCs). This study examined the hypotheses that new myocytes are generated during adolescent growth, to increase myocyte number, and these newly formed myocytes are initially small, mononucleated, proliferation competent, and have immature properties. Ventricular myocytes (VMs) and cKit(+) (stem cell receptor) CS/PCs were isolated from 11- and 22-week feline hearts. Bromodeoxyuridine incorporation (in vivo) and p16(INK4a) immunostaining were measured to assess myocyte cell cycle activity and senescence, respectively. Telomerase activity, contractions, Ca2+ transients, and electrophysiology were compared in small mononucleated (SMMs) and large binucleated (LBMs) myocytes. Heart mass increased by 101% during adolescent growth, but left ventricular myocyte volume only increased by 77%!
Chen, Xiongwen Wilson, Rachel M. Kubo, Hajime Berretta, Remus M. Harris, David M. Zhang, Xiaoying Jaleel, Naser MacDonnell, Scott M. Bearzi, Claudia Tillmanns, Jochen Trofimova, Irina Hosoda, Toru Mosna, Federico Cribbs, Leanne Leri, Annarosa Kajstura, Jan Anversa, Piero Houser, Steven R.