This is an archive of papers published by the staff and faculty of Fox Chase Cancer Center. For questions about content, please contact Talbot Research Library
Last updated on
Dolgikh DA , Latypov RF , Abdullaev ZK , Kolon V , Roder H , Kirpichnikov MP
Expression of mutant genes for horse cytochrome c in Escherichia coli
Bioorganicheskaya Khimiya. 1998 Oct;24(10) :756-759
AbstractHere we describe genetically engineered constructs for the expression in Escherichia coli of genes for horse cytochrome c mutants. These constructs allow the expression of the cytochrome c genes together with heme-ligase, an enzyme which covalently links heme to cytochrome. Careful selection of producer strains and the adjustment of the conditions of expression provided for expression levels of 10-15 mg of protein per liter of culture. This is by an order of magnitude gl eater than the expression previously achieved in yeast. A series of horse cytochrome c mutants were obtained in this way.
NotesTimes Cited: 2 Russian Article 151PX BIOORG KHIM