FCCC LOGO Faculty Publications
Dolgikh DA , Latypov RF , Abdullaev ZK , Kolon V , Roder H , Kirpichnikov MP
Expression of mutant genes for horse cytochrome c in Escherichia coli
Bioorganicheskaya Khimiya. 1998 Oct;24(10) :756-759
PMID: ISI:000077730600004   
Back to previous list
Here we describe genetically engineered constructs for the expression in Escherichia coli of genes for horse cytochrome c mutants. These constructs allow the expression of the cytochrome c genes together with heme-ligase, an enzyme which covalently links heme to cytochrome. Careful selection of producer strains and the adjustment of the conditions of expression provided for expression levels of 10-15 mg of protein per liter of culture. This is by an order of magnitude gl eater than the expression previously achieved in yeast. A series of horse cytochrome c mutants were obtained in this way.
Times Cited: 2 Russian Article 151PX BIOORG KHIM