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Latron F , Jotterand-Bellomo M , Maffei A , Scarpellino L , Bernard M , Strominger JL , Accolla RS
Active suppression of major histocompatibility complex class II gene expression during differentiation from B cells to plasma cells
Proceedings of the National Academy of Sciences of the United States of America. 1988 ;85(7) :2229-2233
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Constitutive expression of major histocompatibility complex class II genes is acquired very early in B-cell ontogeny and is maintained upto the B-cell blast stage. Terminal differentiation in plasma cells is, however, accompanied by a loss of class II gene expression. In B cells this gene system is under the control of several loci encoding transacting factors with activator function, one of which, the aIr-1 gene product, operates across species barriers. In this report human class II gene expression is shown to be extinguished in somatic cell hybrids between the human class II-positive B-cell line Raji and the mouse class II-negative plasmacytoma cell line p3-U1. Since all murine chromosomes are retained in these hybrids and no preferential segregation of a specific human chromosome is observed, the results are compatible with the presence of suppressor factors of mouse origin, operating across species barriers and inhibiting class ii gene expression. Suppression seems to act at the level of transcription or accumulation of class II-specific mRNA, since no human, and very few murine, class II transcripts are detectable in the hybrids.
00278424 (ISSN) Cited By: 21; Export Date: 30 May 2006; Source: Scopus CODEN: PNASA Language of Original Document: English Correspondence Address: Accolla, R.S.; Ludwig Institute for Cancer Research; Lausanne Branch 1066 Epalinges, Switzerland Chemicals/CAS: Histocompatibility Antigens Class II; Transcription Factors