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Bonfil RD , Momiki S , Fridman R , Reich R , Reddel R , Harris CC , Klein-Szanto A
Enhancement of the invasive ability of a transformed human bronchial epithelial cell line by 12-O-tetra-decanoyl-phorbol-13-acetate and diacylglycerol
Carcinogenesis. 1989 ;10(12) :2335-2338
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Abstract
The effect of the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) was studied using an immortalized human bronchial epithelial cell line, BEAS-2B, both in vivo and in vitro. The in vivo model consisted of tracheas reconstituted with an epithelium of BEAS-2B cells xenotransplanted into athymic nude mice. Intraluminal TPA treatment caused increased BEAS-2B cell proliferation and downgrowth into the tracheal stroma. In an in vitro invasion assay, TPA enhanced the invasive capacity of BEAS-2B cells 20- to 25-fold. A similar result was observed with diacylglycerol (DAG), an endogenous activator of protein kinase C, ad the effects of TPA and DAG were abolished by simultaneous treatment with H-7, a protein kinase C inhibitor. TPA induced type IV collagenolysis, and this effect also was prevented by H-7. These data are consistent with the hypothesis that TPA causes these cells to become invasive by inducing collagenase activity and that this effect is mediated via protein kinase C.
Notes
01433334 (ISSN) Cited By: 5; Export Date: 30 May 2006; Source: Scopus CODEN: CRNGD Language of Original Document: English Correspondence Address: Klein-Szanto, A.; Department of Pathology; Fox Chase Cancer Center Philadelphia, PA 19111, United States Chemicals/CAS: phorbol 13 acetate 12 myristate, 16561-29-8, 1,2-dioctanoylglycerol, 1069-87-0; Diglycerides; Glycerides; Microbial Collagenase, EC 3.4.24.3; Tetradecanoylphorbol Acetate, 16561-29-8