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McNeill H , Ozawa M , Kemler R , James Nelson W
Novel function of the cell adhesion molecule uvomorulin as an inducer of cell surface polarity
Cell. 1990 ;62(2) :309-316
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Na+,K+-ATPase has distinctly different distributions in mesenchymal cells, where it has an unrestricted distribution over the entire cell surface, compared with polarized epithelial cells, where it is restricted to the basal-lateral membrane domain. The generation of this restricted distribution is important in mesenchyme to epithelia conversion in development and the function of transporting epithelia, but the mechanisms involved are unknown. Here we show that expression of the epithelial CAM uvomorulin in transfected fibroblasts is sufficient to induce a redistribution of Na+,K+-ATPase to sites of uvomorulin-mediated cell-cell contacts, similar to that in polarized epithelial cells. This restricted distribution of Na+,K+-ATPase occurs in the absence of tight junctions but coincides with the reorganization of the membrane cytoskeleton. The results indicate a direct role for CAMs as inducers of cell surface polarity of selective cytoplasmic and membrane proteins.
00928674 (ISSN) Cited By: 166; Export Date: 30 May 2006; Source: Scopus CODEN: CELLB Language of Original Document: English Correspondence Address: McNeill, H.; Dept. of Molec. and Cell. Physiology; Stanford University; Medical Center Stanford, CA 94305-5426, United States Chemicals/CAS: uvomorulin, 112956-45-3, Cadherins; Calcium, 7440-70-2; Carrier Proteins; Cell Adhesion Molecules; fodrin; Glycoproteins; Microfilament Proteins; Na(+)-K(+)-Exchanging ATPase, EC References: Balcarova-Sta?ndler, J., Pfeiffer, S.E., Fuller, S.D., Simons, K., Development of cell surface polarity in the epithelial cell line Madin-Darby canine (MDCK) cell line (1984) EMBO J., 3, pp. 2141-2150; Bartles, T.R., Feracci, H.M., Stieger, B., Hubbard, A.L., Biogenesis of the rat hepatocyte plasma membrane in vivo: Comparison of the pathways taken by apical and basolateral proteins using subcellular fractionation (1987) J. 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