FCCC LOGO Faculty Publications
Li H , Bera K , Toro P , Fu P , Zhang Z , Lu C , Feldman M , Ganesan S , Goldstein LJ , Davidson NE , Glasgow A , Harbhajanka A , Gilmore H , Madabhushi A
Collagen fiber orientation disorder from H&E images is prognostic for early stage breast cancer: clinical trial validation
NPJ Breast Cancer. 2021 Aug 6;7(1) :104
Back to previous list
Collagen fiber organization has been found to be implicated in breast cancer prognosis. In this study, we evaluated whether computerized features of Collagen Fiber Orientation Disorder in Tumor-associated Stroma (CFOD-TS) on Hematoxylin & Eosin (H&E) slide images were prognostic of Disease Free Survival (DFS) in early stage Estrogen Receptor Positive (ER+) Invasive Breast Cancers (IBC). A Cox regression model named M(CFOD-TS), was constructed using cohort S(t) (Nā€‰=ā€‰78) to predict DFS based on CFOD-TS features. The prognostic performance of M(CFOD-TS) was validated on cohort S(v) (Nā€‰=ā€‰219), a prospective clinical trial dataset (ECOG 2197). M(CFOD-TS) was prognostic of DFS in both S(t) and S(v), independent of clinicopathological variables. Additionally, the molecular pathways regarding cell cycle regulation were identified as being significantly associated with M(CFOD-TS) derived risk scores. Our results also found that collagen fiber organization was more ordered in patients with short DFS. Our study provided a H&E image-based pipeline to derive a potential prognostic biomarker for early stage ER+ IBC without the need of special collagen staining or advanced microscopy techniques.
2374-4677 Li, Haojia Orcid: 0000-0001-5000-5334 Bera, Kaustav Orcid: 0000-0001-9831-6000 Toro, Paula Fu, PingFu Orcid: 0000-0002-2334-5218 Zhang, Zelin Lu, Cheng Feldman, Michael Ganesan, Shridar Goldstein, Lori J Davidson, Nancy E Glasgow, Akisha Harbhajanka, Aparna Gilmore, Hannah Madabhushi, Anant Orcid: 0000-0002-5741-0399 UL1 TR002548/TR/NCATS NIH HHS/United States R01 CA216579/CA/NCI NIH HHS/United States C06 RR012463/RR/NCRR NIH HHS/United States U24 CA199374/CA/NCI NIH HHS/United States R43 EB028736/EB/NIBIB NIH HHS/United States U54 CA254566/CA/NCI NIH HHS/United States U01 CA239055/CA/NCI NIH HHS/United States R01 CA220581/CA/NCI NIH HHS/United States R01 CA202752/CA/NCI NIH HHS/United States R01 CA208236/CA/NCI NIH HHS/United States U01 CA248226/CA/NCI NIH HHS/United States I01 BX004121/BX/BLRD VA/United States Journal Article NPJ Breast Cancer. 2021 Aug 6;7(1):104. doi: 10.1038/s41523-021-00310-z.