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Li H , Bera K , Toro P , Fu P , Zhang Z , Lu C , Feldman M , Ganesan S , Goldstein LJ , Davidson NE , Glasgow A , Harbhajanka A , Gilmore H , Madabhushi A
Collagen fiber orientation disorder from H&E images is prognostic for early stage breast cancer: clinical trial validation
NPJ Breast Cancer. 2021 Aug 6;7(1) :104
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Abstract
Collagen fiber organization has been found to be implicated in breast cancer prognosis. In this study, we evaluated whether computerized features of Collagen Fiber Orientation Disorder in Tumor-associated Stroma (CFOD-TS) on Hematoxylin & Eosin (H&E) slide images were prognostic of Disease Free Survival (DFS) in early stage Estrogen Receptor Positive (ER+) Invasive Breast Cancers (IBC). A Cox regression model named M(CFOD-TS), was constructed using cohort S(t) (Nā€‰=ā€‰78) to predict DFS based on CFOD-TS features. The prognostic performance of M(CFOD-TS) was validated on cohort S(v) (Nā€‰=ā€‰219), a prospective clinical trial dataset (ECOG 2197). M(CFOD-TS) was prognostic of DFS in both S(t) and S(v), independent of clinicopathological variables. Additionally, the molecular pathways regarding cell cycle regulation were identified as being significantly associated with M(CFOD-TS) derived risk scores. Our results also found that collagen fiber organization was more ordered in patients with short DFS. Our study provided a H&E image-based pipeline to derive a potential prognostic biomarker for early stage ER+ IBC without the need of special collagen staining or advanced microscopy techniques.
Notes
2374-4677 Li, Haojia Orcid: 0000-0001-5000-5334 Bera, Kaustav Orcid: 0000-0001-9831-6000 Toro, Paula Fu, PingFu Orcid: 0000-0002-2334-5218 Zhang, Zelin Lu, Cheng Feldman, Michael Ganesan, Shridar Goldstein, Lori J Davidson, Nancy E Glasgow, Akisha Harbhajanka, Aparna Gilmore, Hannah Madabhushi, Anant Orcid: 0000-0002-5741-0399 UL1 TR002548/TR/NCATS NIH HHS/United States R01 CA216579/CA/NCI NIH HHS/United States C06 RR012463/RR/NCRR NIH HHS/United States U24 CA199374/CA/NCI NIH HHS/United States R43 EB028736/EB/NIBIB NIH HHS/United States U54 CA254566/CA/NCI NIH HHS/United States U01 CA239055/CA/NCI NIH HHS/United States R01 CA220581/CA/NCI NIH HHS/United States R01 CA202752/CA/NCI NIH HHS/United States R01 CA208236/CA/NCI NIH HHS/United States U01 CA248226/CA/NCI NIH HHS/United States I01 BX004121/BX/BLRD VA/United States Journal Article NPJ Breast Cancer. 2021 Aug 6;7(1):104. doi: 10.1038/s41523-021-00310-z.