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Zhang T , Ni C , Li C , Lu P , Chen D , Dong Y , Whetstine JR , Zhang Y , Xie Z
Isoflurane impairs oogenesis through germ cell apoptosis in C. elegans
Sci Rep. 2021 Jul 14;11(1) :14481
PMID: 34262087    PMCID: PMC8280124    URL: https://www.ncbi.nlm.nih.gov/pubmed/34262087
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Abstract
Anesthetic isoflurane has been reported to induce toxicity. However, the effects of isoflurane on fecundity remain largely unknown. We established a system in C. elegans to investigate the effects of isoflurane on oogenesis. Synchronized L4 stage C. elegans were treated with 7% isoflurane for 4 h. Dead cells, ROS, embryos, and unfertilized eggs laid by hermaphrodites were measured by fluorescence imaging and counting. The C. elegans with losses of ced-3, cep-1, abl-1, male C. elegans, and oxidative stress inhibitor N-acetyl-cysteine were used in the interaction studies. We found that isoflurane decreased the numbers of embryos and unfertilized eggs and increased the levels of dead cells and ROS in C. elegans. The isoflurane-induced impairment of oogenesis was associated with abl-1, ced-3, but not cep-1. N-acetyl-cysteine attenuated the isoflurane-induced impairment of oogenesis in C. elegans. Mating with male C. elegans did not attenuate the isoflurane-induced changes in oogenesis. These findings suggest that isoflurane may impair oogenesis through abl-1- and ced-3-associated, but not cep-1-associated, germ cell apoptosis and oxidative stress, pending further investigation. These studies will promote more research to determine the potential effects of anesthesia on fecundity.
Notes
2045-2322 Zhang, Tao Ni, Cheng Li, Cheng Lu, Pan Chen, Dan Dong, Yuanlin Whetstine, Johnathan R Zhang, Yiying Xie, Zhongcong R01GM088801/NH/NIH HHS/United States Journal Article Sci Rep. 2021 Jul 14;11(1):14481. doi: 10.1038/s41598-021-93737-0.