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van Eeuwen T , Li T , Kim HJ , Gorbea Colón JJ , Parker MI , Dunbrack RL , Garcia BA , Tsai KL , Murakami K
Structure of TFIIK for phosphorylation of CTD of RNA polymerase II
Sci Adv. 2021 Apr;7(15)
PMID: 33827808    PMCID: PMC8026125    URL: https://www.ncbi.nlm.nih.gov/pubmed/33827808
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During transcription initiation, the general transcription factor TFIIH marks RNA polymerase II by phosphorylating Ser5 of the carboxyl-terminal domain (CTD) of Rpb1, which is followed by extensive modifications coupled to transcription elongation, mRNA processing, and histone dynamics. We have determined a 3.5-Å resolution cryo-electron microscopy (cryo-EM) structure of the TFIIH kinase module (TFIIK in yeast), which is composed of Kin28, Ccl1, and Tfb3, yeast homologs of CDK7, cyclin H, and MAT1, respectively. The carboxyl-terminal region of Tfb3 was lying at the edge of catalytic cleft of Kin28, where a conserved Tfb3 helix served to stabilize the activation loop in its active conformation. By combining the structure of TFIIK with the previous cryo-EM structure of the preinitiation complex, we extend the previously proposed model of the CTD path to the active site of TFIIK.
2375-2548 van Eeuwen, Trevor Orcid: 0000-0003-0549-1702 Li, Tao Orcid: 0000-0003-3672-6906 Kim, Hee Jong Orcid: 0000-0002-0823-5301 Gorbea Colón, Jose J Orcid: 0000-0002-6327-5672 Parker, Mitchell I Orcid: 0000-0003-1223-3904 Dunbrack, Roland L Orcid: 0000-0001-7674-6667 Garcia, Benjamin A Tsai, Kuang-Lei Orcid: 0000-0002-5428-4219 Murakami, Kenji Orcid: 0000-0002-2017-5575 Journal Article Sci Adv. 2021 Apr 7;7(15):eabd4420. doi: 10.1126/sciadv.abd4420. Print 2021 Apr.