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Baraniya D , Chen T , Nahar A , Alakwaa F , Hill J , Tellez M , Ismail A , Puri S , Al-Hebshi NN
Supragingival mycobiome and inter-kingdom interactions in dental caries
J Oral Microbiol. 2020 ;12(1) :1729305
PMID: 32158514    PMCID: PMC7048226   
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Abstract
Background: Recent studies have reveled the presence of a complex fungal community (mycobiome) in the oral cavity. However, the role of oral mycobiome in dental caries and its interaction with caries-associated bacteria is not yet clear. Methods: Whole-mouth supragingival plaque samples from 30 children (6-10 years old) with no caries, early caries, or advanced caries were sequenced for internal transcribed spacer 2 (ITS-2). The mycobiome profiles were correlated with previously published bacteriome counterparts. Interaction among selected fungal and bacterial species was assessed by co-culture or spent media experiments. Results: Fungal load was extremely low. Candida, Malassezia, Cryptococcus, and Trichoderma spp. were the most prevalent/abundant taxa. Advanced caries was associated with significantly higher fungal load and prevalence/abundance of Candida albicans. Cryptococcus neoformans and Candida sake were significantly over-abundant in early caries, while Malassezia globosa was significantly enriched in caries-free subjects. C. albicans correlated with Streptococcus mutans and Scardovia wiggsiae among other caries-associated bacteria, while M. globosa inversely correlated with caries-associated bacteria. In-vitro, M. globosa demonstrated inhibitory properties against S. mutans. Conclusions: the results substantiate the potential role of the oral mycobiome, primarily Candida species, in dental caries. Inter-kingdom correlations and inhibition of S. mutans by M. globosa are worth further investigation.
Notes
Baraniya, Divyashri Chen, Tsute Nahar, Anubhav Alakwaa, Fadhl Hill, Jennifer Tellez, Marisol Ismail, Amid Puri, Sumant Al-Hebshi, Nezar Noor Journal Article United States J Oral Microbiol. 2020 Feb 19;12(1):1729305. doi: 10.1080/20002297.2020.1729305. eCollection 2020.