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Al-Hebshi NN , Baraniya D , Chen T , Hill J , Puri S , Tellez M , Hasan NA , Colwell RR , Ismail A
Metagenome sequencing-based strain-level and functional characterization of supragingival microbiome associated with dental caries in children
J Oral Microbiol. 2019 ;11(1) :1557986
PMID: 30671194    PMCID: PMC6327923   
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Abstract
Studies of the microbiome associated with dental caries have largely relied on 16S rRNA sequence analysis, which is associated with PCR biases, low taxonomic resolution, and inability to accurately study functions. Here, we employed whole metagenome shotgun sequencing, coupled with high-resolution analysis algorithm, to analyze supragingival microbiomes from 30 children with or without dental caries. A total of 726 bacterial strains belonging to 406 species, in addition to 34 bacteriophages were identified. A core bacteriome was identified at the species and strain levels. Species of Prevotella, Veillonella, as yet unnamed Actinomyces, and Atopobium showed strongest association with caries; Streptococcus sp. AS14 and Leptotrichia sp. Oral taxon 225, among others, were overabundant in caries-free. For several species, the association was strain-specific. Furthermore, for some species, e.g. Streptococcus mitis and Streptococcus sanguinis, sister strains showed differential associations. Noteworthy, associations were also identified for phages: Streptococcus phage M102 with caries and Haemophilus phage HP1 with caries-free. Functionally, potentially relevant features were identified including urate, vitamin K2, and polyamine biosynthesis in association with caries; and three deiminases and lactate dehydrogenase with health. The results demonstrate new associations between the microbiome and dental caries at the strain and functional levels that need further investigation.
Notes
Al-Hebshi, Nezar Noor Baraniya, Divyashri Chen, Tsute Hill, Jennifer Puri, Sumant Orcid: 0000-0002-5509-007x Tellez, Marisol Hasan, Nur A Colwell, Rita R Ismail, Amid Journal Article United States J Oral Microbiol. 2018 Dec 28;11(1):1557986. doi: 10.1080/20002297.2018.1557986. eCollection 2019.