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Sultanov DC , Gerasimova NS , Kudryashova KS , Maluchenko NV , Kotova EY , Langelier MF , Pascal JM , Kirpichnikov MP , Feofanov AV , Studitsky VM
Unfolding of core nucleosomes by PARP-1 revealed by spFRET microscopy
AIMS Genet. 2017 Jan;4(1) :21-31
PMID: 28804761    PMCID: PMC5552189    URL: https://www.ncbi.nlm.nih.gov/pubmed/28804761
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DNA accessibility to various protein complexes is essential for various processes in the cell and is affected by nucleosome structure and dynamics. Protein factor PARP-1 (poly(ADP-ribose)polymerase 1) increases the accessibility of DNA in chromatin to repair proteins and transcriptional machinery, but the mechanism and extent of this chromatin reorganization are unknown. Here we report on the effects of PARP-1 on single nucleosomes revealed by spFRET (single-particle Forster Resonance Energy Transfer) microscopy. PARP-1 binding to a double-strand break in the vicinity of a nucleosome results in a significant increase of the distance between the adjacent gyres of nucleosomal DNA. This partial uncoiling of the entire nucleosomal DNA occurs without apparent loss of histones and is reversed after poly(ADP)-ribosylation of PARP-1. Thus PARP-1-nucleosome interactions result in reversible, partial uncoiling of the entire nucleosomal DNA.
Sultanov, Daniel C Gerasimova, Nadezhda S Kudryashova, Kseniya S Maluchenko, Natalya V Kotova, Elena Y Langelier, Marie-France Pascal, John M Kirpichnikov, Mikhail P Feofanov, Alexey V Studitsky, Vasily M R01 GM058650/GM/NIGMS NIH HHS/United States R01 GM087282/GM/NIGMS NIH HHS/United States Journal Article United States AIMS Genet. 2017;4(1):21-31. doi: 10.3934/genet.2017.1.21. Epub 2017 Jan 5.