FCCC LOGO Faculty Publications
Volokh OI , Derkacheva NI , Studitsky VM , Sokolova OS
[Structural studies of chromatin remodeling factors]
Mol Biol (Mosk). 2016 Nov-Dec;50(6) :922-934
PMID: 28064308   
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Abstract
Changes of chromatin structure require participation of chromatin remodeling factors (CRFs), which are ATP-dependent multisubunit complexes that change the structure of the nucleosome without covalently modifying its components. CRFs act together with other protein factors to regulate the extent of chromatin condensation. Four CRF families are currently distinguished based on their structural and biochemical characteristics: SWI/SNF, ISWI, Mi-2/CHD, and SWR/INO80. X-ray diffraction analysis and electron microscopy are the main methods to obtain structural information about macromolecules. CRFs are difficult to obtain in crystal because of their large sizes and structural heterogeneity, and transmission electron microscopy (TEM) is mostly employed in their structural studies. The review considers all structures obtained for CRFs by TEM and discusses several models of CRF-nucleosome interactions.
Notes
Volokh, O I Derkacheva, N I Studitsky, V M Sokolova, O S Journal Article Review Russia (Federation) Mol Biol (Mosk). 2016 Nov-Dec;50(6):922-934. doi: 10.7868/S0026898416060215.