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Studitsky VM , Nizovtseva EV , Shaytan AK , Luse DS
Nucleosomal Barrier to Transcription: Structural Determinants and Changes in Chromatin Structure
Biochem Mol Biol J. 2016 ;2(2)
PMID: 27754494    PMCID: PMC5041593    URL: https://www.ncbi.nlm.nih.gov/pubmed/27754494
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Packaging of DNA into chromatin affects all processes on DNA. Nucleosomes present a strong barrier to transcription, raising important questions about the nature and the mechanisms of overcoming the barrier. Recently it was shown that DNA sequence, DNA-histone interactions and backtracking by RNA polymerase II (Pol II) all contribute to formation of the barrier. After partial uncoiling of nucleosomal DNA from histone octamer by Pol II and backtracking of the enzyme, nucleosomal DNA recoils on the octamer, locking Pol II in the arrested state. Histone chaperones and transcription factors TFIIS, TFIIF and FACT facilitate transcription through chromatin using different molecular mechanisms.
Studitsky, Vasily M Nizovtseva, Ekaterina V Shaytan, Alexey K Luse, Donal S R01 GM058650/GM/NIGMS NIH HHS/United States Journal article United States Biochem Mol Biol J. 2016;2(2). pii: 8. Epub 2016 May 30.