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Yang J , Zhu L , Zhang H , Hirbawi J , Fukuda K , Dwivedi P , Liu J , Byzova T , Plow EF , Wu J , Qin J
Conformational activation of talin by RIAM triggers integrin-mediated cell adhesion
Nat Commun. 2014 Dec 18;5 :5880
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The membrane localization and activation of cytoskeletal protein talin are key steps to initiate the integrin transmembrane receptors' activation, which mediates many cellular adhesive responses such as cell migration, spreading and proliferation. RIAM, a membrane anchor and small GTPase RAP1 effector, is known to bind to the C-terminal rod domain of talin (talin-R) and promote localizations of talin to the membrane. Through systematic mapping analysis, we find that RIAM also binds to the N-terminal head of talin (talin-H), a crucial domain involved in binding and activating integrins. We show that the RIAM binding to talin-H sterically occludes the binding of a talin-R domain that otherwise masks the integrin-binding site on talin-H. We further provide functional evidence that such RIAM-mediated steric unmasking of talin triggers integrin activation. Our findings thus uncover a novel role for RIAM in conformational regulation of talin during integrin activation and cell adhesion.
2041-1723 Yang, Jun Zhu, Liang Zhang, Hao Hirbawi, Jamila Fukuda, Koichi Dwivedi, Pallavi Liu, Jianmin Byzova, Tatiana Plow, Edward F Wu, Jinhua Qin, Jun P01 HL073311/HL/NHLBI NIH HHS/United States R01 HL096062/HL/NHLBI NIH HHS/United States Journal Article England Nat Commun. 2014 Dec 18;5:5880. doi: 10.1038/ncomms6880.