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Haugh KA , Shalginskikh N , Nogusa S , Skalka AM , Katz RA , Balachandran S
The interferon-inducible antiviral protein Daxx is not essential for interferon-mediated protection against avian sarcoma virus
Virol J. 2014 ;11(1) :100
PMID: 24884573    PMCID: PMC4049388   
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BACKGROUND: The antiviral protein Daxx acts as a restriction factor of avian sarcoma virus (ASV; Retroviridae) in mammalian cells by promoting epigenetic silencing of integrated proviral DNA. Although Daxx is encoded by a type I (alpha/beta) interferon-stimulated gene, the requirement for Daxx in the interferon anti-retroviral response has not been elucidated. In this report, we describe the results of experiments designed to investigate the role of Daxx in the type I interferon-induced anti-ASV response. FINDINGS: Using an ASV reporter system, we show that type I interferons are potent inhibitors of ASV replication. We demonstrate that, while Daxx is necessary to silence ASV gene expression in the absence of interferons, type I interferons are fully-capable of inducing an antiviral state in the absence of Daxx. CONCLUSIONS: These results provide evidence that Daxx is not essential for the anti-ASV interferon response in mammalian cells, and that interferons deploy multiple, redundant antiviral mechanisms to protect cells from ASV.
1743-422x Haugh, Kelsey A Shalginskikh, Natalia Nogusa, Shoko Skalka, Anna Marie Katz, Richard A Balachandran, Siddharth Journal Article England Virol J. 2014 May 28;11(1):100. doi: 10.1186/1743-422X-11-100.