Lattice_grid_med
Powered by LatticeGrid

Search Enter term and hit return. Use '*' for as a wildcard.
Pan ZZ, Bruening W, Godwin AK
Involvement of RHO GTPases and ERK in synuclein-gamma enhanced cancer cell motility
INTERNATIONAL JOURNAL OF ONCOLOGY (2006) 29:1201-1205.
Abstract
Synuclein-gamma is aberrantly expressed in more than 70% of stage III/IV breast and ovarian carcinomas. Ectopic overexpression of synuclein-gamma enhanced MDA-MB-435 cell migration in vitro and metastasis in a nude mouse model. However, the mechanism of how synuclein-gamma promotes cell motility is not clear. In our previous studies, we showed that synuclein-gamma overexpression activates ERK. In the present study, we overexpressed synuclein-y in several breast and ovarian cancer cell lines and evaluated the effect of synuclein-y on the activity of small G-protein RHO family members. We found that at least one of the RHO/RAC/CDC42 GTPases showed a higher level of the GTP-bound active form. Consistent with their role in regulating the intracellular motile machinery, inhibition of the RHO/RAC/CDC42 by C. difficile Toxin B blocked cell migration in both parental cells and synuclein-gamma overexpressing cells. The ERK inhibitor U0126 also blocked the cell migration in both paren! tal cells and synuclein-y overexpressing cells. Collectively, our data indicate that synuclein-y might be involved in late stage breast and ovarian cancer metastasis by enhancing cell motility through activation of the RHO family small-GTPases and ERK.
Note
Publication Date: 2006-11-01.
Back
Last updated on Wednesday, March 04, 2020