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Peripheral CD4(+) T cell maturation recognized by increased expression of Thy-1/CD90 bearing the 6C10 carbohydrate epitope
Journal of Immunology (1999) 163:4796-4804.
Abstract
The SPV16C10 IgM autoantibody recognizes a surface determinant, 6C10, that is highly expressed on all immature thymocytes, In contrast, its expression on peripheral T cells appears developmentally regulated, i.e., absent from most naive T cells in spleen of neonatal mice, but expressed on 40-80% of naive CD4(+) T cells in adult. In this paper, we demonstrate that SM6C10 recognizes a carbohydrate epitope on the Thy-1 glycoprotein using immunoprecipitation analysis, by binding to affinity-purified Thy-1 in an ELISA, and by sensitivity to N- glycosidase-F treatment. Retroviral Thy-1 gene transduction experiments into Thy-1(-) variant T cell lines and a pro-B cell line provide evidence that 6C10 glycosylated Thy-1 expression is not restricted to T cells but depends on the recipient cell. Therefore, differences in 6C10 levels among Thy-1(+) T cells in mice likely reflect developmental regulation of posttranslational modification of the Thy-1 glycoprotein, The ability of naive CD4(+) T cells to respond to anti-Thy-1 stimulation increases from neonate to adult, and 6C10(-) naive cells from adult mice respond poorly compared with 6C10(+) cells, similar to the cells in neonatal mice. These results suggest that there is functional maturation by peripheral CD4(+) T cells that coincides with 6C10 glycosylated Thy-1 up- regulation, and natural autoantibody recognizes this 6C10 carbohydrate epitope.
Note
Publication Date: 1999-11-01.
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