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Golemis EA, Serebriiskii I, Finley RL, Kolonin MG, Gyuris J, Brent R
Interaction trap/two-hybrid system to identify interacting proteins
Curr Protoc Cell Biol (2011) Chapter 17:Unit17 3.
Abstract
The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins. Curr. Protoc. Cell Biol. 53:17.3.1-17.3.35. (c) 2011 by John Wiley & Sons, Inc.
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Publication Date: 2011-12-01.
PMCID: not NIH funded
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