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Zaret K
Micrococcal nuclease analysis of chromatin structure
Curr Protoc Mol Biol (2005) Chapter 21:Unit 21 1.
Abstract
This unit describes methodology for using micrococcal nuclease to investigate the presence of nucleosomes at a particular location in chromatin and to map the positions of nucleosomes at various levels of resolution. The approaches are readily adaptable to other probes of chromatin structure that cause DNA cleavage. Results obtained from such chromatin studies provide a structural view of the molecular environment of gene in their native context in cells.
Note
Publication Date: 2005-02-01.
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Last updated on Friday, August 07, 2020