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Wen L, Shinton SA, Hardy RR, Hayakawa K
Association of B-1 B Cells with Follicular Dendritic Cells in Spleen
Journal of Immunology (2005) 174:6918-6926.
Although CD5+ B-1 B cells have been recognized as an infrequent B cell subset in mice for many years, attempts to identify their histol. location in normal mouse spleen have proven difficult due to both their paucity and low level expression of CD5. In this study the authors have studied VH11/DH/JH gene-targeted mice, VH11t, that develop elevated nos. of CD5+ VH11/Vk9 B cells with an anti-phosphatidylcholine (anti-PtC) autoreactive specificity, allowing B-1 B cell detection by anti-PtC Id-specific Abs in spleen section staining. Using this approach the authors found that anti-PtC B-1 cells first appear within the white pulp in neonates, expand in assocn. with follicular dendritic cells (FDC), and localize more centrally than other (non-B-1) IgDhigh follicular B cells in adults. Among neonatal B cells, CD5+ B-1 cells in both normal and VH11t mouse spleen and peritoneal cavity express the highest levels of CXCR5, which is important for FDC development. Injection of purified spleen or peritoneal B-1 cells into RAG knockout mice resulted in B-1 cell follicle formation in spleen, inducing FDC development and plasma cell generation. These results indicate that B-1 B cells are the first B cells to express fully mature levels of CXCR5, thereby promoting the development of FDC. [on SciFinder (R)]
Publication Date: 2005-01-01.
Last updated on Tuesday, June 05, 2018